ABSTRACT
Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contaminated oysters. This study describes the application of a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerase-coding region of the norovirus genome, and DNA sequencing for the detection and preliminary characterisation of noroviruses in outbreaks of gastroenteritis in children in Brazil. The outbreak samples were collected from children <5 years of age at the Bertha Lutz children's day care facility at Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, that occurred between 1996 and 1998, where no pathogen had been identified. At the Bertha Lutz day care center facility, only Fiocruz's employee children are provided for, and they come from different social, economic and cultural backgrounds. Three distinct genogroup II strains were detected in three outbreaks in 1997/98 and were most closely related to genotypes GII-3 (Mexico virus) and GII-4 (Grimsby virus), both of which have been detected in paediatric and adult outbreaks of gastroenteritis worldwide.
Subject(s)
Humans , Child, Preschool , Child , Caliciviridae , Disease Outbreaks , Gastroenteritis , Acute Disease , Brazil , Caliciviridae , Child Day Care Centers , Feces , Gastroenteritis , Genotype , Reverse Transcriptase Polymerase Chain Reaction , RNA, ViralABSTRACT
Neste estudo, a produçäo de toxinas por 91 amostras de E. coli, isoladas de leitöes com colibacilose, foi testada em cultivo de células VERO. Observou-se que 20 (22,0 por cento) das amostras foram citotóxicas e seis (6,6 por cento) foram citotônicas; somente uma amostra foi citotóxica e citotônica simultaneamente. Sete (87,5 por cento) das amostras isoladas de doença do edema produziram verotoxina. Todas as amostras citotônicas foram inativadas à 60§C/15 min e as citotóxicas, à 70§C/15 min